منابع مشابه
Lipid metabolism in cultured cells.
Utilization of serum triglycerides by cells in tissue culture has been studied in order to determine their importance as a source of cell lipids and to obtain information on the cellular mechanisms of triglyceride uptake. L strain mouse fibroblasts incorporated serum triglycerides from the growth medium, but the rates of uptake were up to IO-fold less than that of free fatty acids or monoglycer...
متن کاملLipid metabolism in cultured cells. Activators of endogenous thromboxane A2 synthesis in cultured lung fibroblasts.
Thromboxane A2 (rabbit aorta-contracting substance) is a proaggregatory vasoconstrictive, oxygenated metabolite of arachidonic acid which was originally discovered in guinea pig lung perfusates during antigen-induced anaphylaxis. The specific stimuli which activate synthesis and the cellular source in the lung remain undefined. In order to study pulmonary thromboxane A2 (TXA2) synthesis, a cult...
متن کاملLipid metabolism in cultured cells: growth of tumor cells deficient in essential fatty acids.
di-cis-decadienyl structure are apparently required for maintenance of normal growth and reproduction (16). The commonly occurring fatty acids exhibiting this structural moiety are linoleic (18 :2w6)', linolenic (1 8:3w3), and arachidonic acids (20:4co6). Since they are not synthesized de novo, they have been termed the â€oeEFA's.†• 2 A dietary requirement for these fatty acids has been shown...
متن کاملEffect of ethanol on lipid metabolism in cultured hepatocytes.
Isolated rat hepatocytes were cultured in a modified HI-WO/BA medium for 16 h. In the following 24 h oleate or oleate plus ethanol was added to the medium. After this period the medium was changed again and the cultures were further incubated with [1-14C]oleate alone or with [1-14C]oleate plus ethanol for 6 h. This allowed a comparison of effects of short-term (6 h) and long-term (24 + 6 h) exp...
متن کاملCarbohydrate Metabolism in Cultured Cells 813
The me tabo l i sm of radioact ively labelled D-glucose, L-glutamine, and L-glutamate has been determined in a g l y c o l y t i c mu t an t of C h i n e s e h a m s t e r ovary cells, R l . I . 7 , and in its parent, CHO-K1. The complete oxidation of glucose via the TCA-cycle is negligible in both cell types, but there is significant oxidation of carbon-1. CHO-K1 cells derive most of their ene...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44288-9